Gene sequencing

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1. Summary Deoxyribonucleic Acid Watson and Crick model DNA Sequencing Purpose DNA Sequencing Method Maxam & Gilbert Method Sanger Method Comparison Application of…
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  • 1. Summary Deoxyribonucleic Acid Watson and Crick model DNA Sequencing Purpose DNA Sequencing Method Maxam & Gilbert Method Sanger Method Comparison Application of DNA Sequencing Advantages & Disadvantages
  • 2. Shotgun sequencing
  • 3. Direct approach Direct approach
  • 4. Next generation DNA sequencing Next generation sequencing platforms differ from traditional Sanger sequencing technology in a number of ways. First, the DNA sequencing libraries are clonally amplified in vitro, obviating the need for time consuming and laborious cloning of the DNA library into bacteria Second, the DNA is sequenced by synthesis, such that the DNA sequence is determined by the addition of nucleotides to the complementary strand rather through chain termination chemistry. Finally, the spatially segregated, amplified DNA templates are sequenced simultaneously in a massively parallel fashion without the requirement for a physical separation step.
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